- 企业认证： 定制 备
- 注册资本：100万元（人民币）
- 企业性质：私营独资企业
- 主营产品：1.进口胎牛血清：美国GIBCO、德国PAN-biotech、以色列BI、美国GE..
- 公司地址：北京市海淀区西北旺唐家岭村南
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- 产地： 美国
- 品牌： Roche
- 货号： 10814245001
- 英文名称： Restr.-Endonucl. Asp 718, 1000
- 保质期： 见包装 个月
- 保存条件： -20℃
Restr.-Endonucl. Asp 718, 1000
The buffer in bold is recommended for optimal activity
? A: 75-100%
? B: 100%
? H: 50-75%
? L: 0-10%
? M: 25-50%
Activity in PCR buffer: 100%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained λDNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.
Compatible ends
Asp718 I ends are compatible with ends generated by Ban I , Bsi W I and SspB I.
Isoschizomers
The enzyme is an isoschizomer of Acc65 I and Kpn I.
Methylation sensitivity
Asp718 I is inhibited by dcm methylation at the site indicated (*) on the recognition sequence. Simultaneousmethylation of A(°) and the most central C does not influence enzyme activity.
Ligation and recutting assay
Asp718 I fragments obtained by complete digestion of 1 μg λDNA are ligated with 1 U T4 DNA Ligase in a volume of 10 μl by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithioerythritol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >90% recovery of λDNA.
Subsequent re-cutting with Asp718 I yields >90% of the typical pattern of λDNA ×Asp718 I fragments.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Quality
Absence of nonspecific endonuclease activities
1 μg λDNA is incubated for 16 hours in 50 μl SuRE/Cut Buffer B with an excess of Asp718 I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Asp718 I for 4 hours at +37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Specificity
Recognition sites: GGT°AC*C
GGT°AC*C
Restriction site: G↓GT°AC*C
G↓GT°AC*C
Heat inactivation: There is no information available whether or not Asp718 I can be heat inactivated.
Unit Definition
One unit is the enzyme activity that completely cleaves 1 μg λDNA in one hour at+37 °C in a total volume of 25 μl (1x) SuRE/Cut Buffer B.
DNA Profile
Number of cleavage sites on different DNAs
? λ: 2
? φX174: 0
? Ad2: 8
? M13mp7: 0
? pBR322: 0
? pBR328: 0
? pUC18: 1
? SV40: 1
性质
| Related Categories | Restriction Enzymes, 分子生物学, 分子生物学酶 更多... |
| form | solution |
| mfr. no. | Roche |
| packaging | pkg of 1,000 U (10814245001 [10 U/μl]) |
| pkg of 5,000 U (10814253001 [10 U/μl]) | |
| pkg of 5,000 U (11175050001 [40 U/μl]) | |
| limit | 37 °C optimum reaction temp. |
| shipped in | dry ice |
| storage temp. | ?20°C |
北京智杰方远科技有限公司成立于2011年，是一家进口胎牛血清专业供应商,专注细胞生物学试剂开发、销售、服务的高新技术企业。主要代理产品如下： 1.进口胎牛血清：美国GIBCO、德国PAN-biotech、以色列BI、美国GEMINI、美国Hyclone等。 2.OriGen细胞冻存产品，TAKARA、Biolog微生物鉴定板、梅里埃试剂、Borer清洗液、台湾旭基产品。 3.Thermo、SIGMA、QIAGEN、MERCK、LEICA、Millipore、Whatman、MP Bio、日本同仁等。好产品+好价格+好服务为经营理念，希望不断发展的智杰方远成为您 的选择！
| 成立时间： | 2011-08-15 | 企业经济性质： | 私营独资企业 | 年营业额： | 100万-250万 |
| 注册资金： | 100万元(人民币) | 经营模式： | 贸易商,代理商, | 员工人数： | 5-10 |
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