您好, 盖德化工网欢迎您! 请登录 免费注册
产品中心
产品
  • 产品
  • 企业
  • 求购
  • 字典
  • 资讯
  • 展会
  • 路线
委托采购
产品
  • 产品
  • 企业
  • 求购
  • 字典
  • 资讯
  • 展会
  • 路线
委托采购

血蓝蛋白/KLH 主营

  面议
起批量 ≥1 10mg
最小起订110mg
供货总量100010mg
发货地址广东省深圳市
建议售价¥面议
更新日期2021年06月11日
VIP5年
企业认证: 定制
企业性质:私营独资企业
主营产品:细胞,抗体抗原,多肽,试剂盒,血蓝蛋白,荧光金,诊断抗体抗原..
公司地址:深圳市南山区创业路中兴工业城5栋518
手机查看
手机查看
一键电话致商家
产地: 美国
品牌: Biosyn
货号: KLH-NP
英文名称: Keyhole Limpet Hemocyanin/KLH
保质期: 4年 个月
保存条件: 2-8℃


血蓝蛋白(KLH)来源于大锁孔帽贝(透孔螺)的血淋巴,也被叫做匙孔血蓝蛋白,是具有高度免疫原性的蛋白大分子,也是一种多功能蛋白,它不仅具有输氧功能,而且还与能量的贮存、渗透压的维持及蜕皮过程的调节有关,还具有酚氧化物酶活性和抗菌功能,被认为是节肢动物和软体动物中的一种重要的免疫分子。
KLH广泛作为载体蛋白应用,交联于半抗原和其他抗原,使它们具有更强的免疫原性以用于抗体的制备。由于其质量大,复杂性高,与其他载体蛋白相比KLH会引出更强的免疫应答。因为此蛋白来源于软体动物,它在系统发生上远离哺乳动物物种,在测定过程中产生会与典型靶点样品发生交叉反应的抗体的可能性较小。作为大的蛋白质,KLH含有上百个伯胺和羧基基团可以成为与戊二醛,N-羟基琥珀酰亚胺酯和EDC等交联试剂交联的靶点。
传统冻干粉状态血蓝蛋白(KLH)会产生大量沉淀并可能导致在使用该产品过滤沉淀时损失高达30%-40%, 好的血蓝蛋白(KLH)制剂溶液应在结合前极少沉淀,从而可产生高效的偶联和增强抗体反应。

高分子量的天然KLH在近些年中得到了广泛的应用。但是大多数可获得的KLH配方并不适合在人体临床试验中使用。通常这些产品中的内毒素含量很高,因为血淋巴血清的生产是从冷冻动物中提取来完成的,在冻干KLH过程中可能导致沉淀产生。部分文献表明,冷冻干燥天然KLH都会导致活性的丧失。
为了KLH产品的安全性、质量和一致性,Biosyn开发了非致命性血淋巴血清采集程序,并制定了必要的动物处理和检疫技术,制定了这种关键原料规模生产的质量控制方法,在磷酸盐缓冲液中生产低内毒素和高浓度天然KLH。科研级别Native KLH是最常用的免疫载体蛋白,适用于生物活性测定、免疫学研究;同时也可用于制备多肽抗原,以进行免疫和抗体生产,疫苗产品开发等项目。

 

与同类产品相比,biosyn公司KLH产品具有以下特点:
*的捕获和处理活动物的流程,不使用冷冻动物等致命提取方法,有利于KLH分子保持稳定。
在缓冲液体系中配制,比其他冻干粉产品具有更好的溶解性。
诊断级别KLH严格通过生物安全性和包括甲乙丙型肝炎、弧菌、轮状病毒、诺沃克病毒在内的无病毒检测。
高分子量,高免疫原性(400kDa)

 

Biosyn公司于1995年创立于美国加州,是一家专业研发和生产高质量的科研和临床级别血蓝蛋白的生物医药公司。目前biosyn公司生产的血蓝蛋白在大型国际医药公司中有约30%的市场占有率。biosyn公司的GMP级产品IMMUCOTHEL®率先于1997获得荷兰相关批文,而后也相继被韩国、阿根廷、奥地利批准进入市场。biosyn公司另一产品VACMUNE®也已于2007年向美国FDA提交了DMF文件。

biosyn公司在海水中养殖的软体动物大锁孔帽贝;在GMP级别厂房生产的KLH能提供极好的免疫原性,以及出色的稳定性和溶解性。

 

KLH产品部分文献:

Henry Milgrom, Int Arch Allergy Immunol. 2012 Feb; 157(3): 269–274. 2011 Oct 28.  PMID: 22042247.
Federico Baruffaldi,Mol Pharm. 2018 Nov 5; 15(11): 4947–4962. PMID: 30240216.
Ashwin Swaminathan, Br J Clin Pharmacol. 2014 Nov; 78(5): 1135–1142. PMID: 24833186.
Jeffrey S.Miller. Clinical Immunology. Volume 117, Issue 2, November 2005, P144-151.
Brendan D. Curti, Cancer Research.  December 2013.
Rong Shi PhD  Marek Honczarenko MD, PhD.  Clinical Pharmacology. 12 July 2016. 
Willemijn Hobo, Frans Maas, Blood 2010 116:4501-4511.
Michal Radomski, Herbert J. Zeh. Journal for ImmunoTherapy of Cancer. 19 April 2016.
 

KLH: Keyhole Limpet Hemocyanin
The hemocyanin produced from the hemolymph sera of the marine mollusk Giant Keyhole Limpet, Megathura crenulata, has been in use and marketed as a crude or partially purified product for over 40 years by some chemical companies. This molecule is well recognized and commonly abbreviated as KLH. The crude research grade KLH is used in antibody production in animals against antigens. The animal sera containing the antibody for the antigen are further processed and used as immunological reagents or in immunological assays. The partially purified product has also been used in early human trials for immune status evaluation.

KLH structure
The native KLH is a cylindrical copper containing blue protein with a molecular mass ranging from 8-32 MDa (Million Dalton). Electron microscopy structural studies reveal presence of two oligomeric forms:

Didecamers with a molecular mass of 8 MDa, are present in the form of hollow cylinders. They are approximately 35 nm in diameter and 40 nm in length.
Multidecamers with a molecular mass of 12-32 MDa, present as stacked decamers (multidecamers) of varying length, added on one or both sides of a "nucleating" didecamer. Single decamers exhibit rectangular side views.
Keyhole Limpet Hemocyanin Structure


Prof. Dr. Jürgen Markl, Direktor Zoologisches Institut der Johannes Gutenberg-Universit?t Mainz, Germany
The didecamer consists of 20 subunits and occurs in two different Isoforms named KLH1 and KLH2. The subunits are biochemically and immunologically distinct. Analysis of KLH by native-PAGE gives two characteristic bands, one corresponding to KLH1 with an apparent molecular mass of about 390 kDa (kilo Dalton) and the other corresponding to KLH2 with an apparent molecular mass of 350 kDa.

biosyn native KLH, low enodoxin content
The high molecular weight, native KLH, is extensively used and has been used over the last several years. Native KLH has been manufactured and supplied by some chemical suppliers. 

However, most available KLH formulations are not of the required quality and consistency suitable for use in human clinical trials. Typically, the endotoxin content in such products is very high as the hemolymph sera production is done by cutting open the animals or extraction from frozen animals. The product characteristics are also not very good and could lead to precipitation during reconstitution of lyophilized KLH. It is well documented that either freezing or lyophilization of native KLH leads to loss in activity. 

Characterization of native KLH is also an issue as the large molecular weight of 8-32 MDa does not allow use of standard biochemical methods for routine quality control, which is further complicated by the use of excessive metal ions like calcium and magnesium, presumably to stabilize the molecule. 

Right from the start of the hemocyanin development work in 1985, biosyn had identified these to be critical issues and developed appropriate strategies to overcome these limitations. To achieve the desired safety, quality and consistency of native KLH, biosyn has developed the non-lethal hemolymph sera collection procedure from live animals and instituted necessary animal handling and quarantine techniques. Quality control methodologies for routine production of this critical raw material has been developed. 

The end result of these technology developments in the animal handling, bleeding and manufacturing process was the production of biosyn low endotoxin and high concentration native KLH of consistent high quality in a phosphate buffer.

The biosyn native KLH is supplied as High Purity Grade and Research Grade (no endotoxin testing). The GMP grade material is ideally suited for use as a carrier protein in the manufacture of vaccines for human use.  It is tested for biological safety, viral clearance and heavy metal ions.

The Research Grade material is suited for use in vaccine product development activities and also for routine immunological studies, antibody production, production of activated KLH and other developmental activities. The native KLH formulations currently available from biosyn are listed in the table.

ProductGradeUses
Native KLH in Phosphate buffer low endotoxin, tested for biological safety, viral clearance and metal ionsGMP
Clinical
grade
For further processing in KLH-conjugate vaccine manufacture for human and animal use
Native KLH in Phosphate buffer, or other buffers low endotoxinRGRoutine immunological and antibody studies and antibody production, initial product development activity for conjugate vaccine. Preparation of Research Grade activated KLH
Native KLH in Phosphate buffer or other buffers. No endotoxin testingRGLow cost research grade material for research uses

RG = Research Grade

 

Why use biosyn KLH?
biosyn KLH has many advantages over other commercial grade material that can be procured from other vendors. The major distinction lies in biosyn's:

Environmentally friendly process used in the extraction of hemocyanin from animals.
biosyn returns the animals back to its natural habitat, as it is a humane approach as well ensures that the animals recovers readily and more impotantly assures the product quality and reproducibility leading to no potential undefined, difficult to characterize, structural alterations to the hemocyanin molecule.
biosyn uses a proprietary methodology and has built-in a test procedure for harvesting and handling of live animals, unlike the use of dead or frozen animals or lethal extraction procedure used by some commercial KLH suppliers.
biosyn hemocynin and final products are therefore of high purity and low endotoxin quality.
biosyn KLH subunits, immunocyanin, are of low molecular weight (400 kDa) and are a completely characterized product.
biosyn manufactures and packages KLH products to meet specific customer requirements including userfriendly, ready-to-use preparation and packaging.
biosyn hemocyanin and immunocyanin are formulated in either pure buffer systems like phosphate buffer or in water and packaged as small aliquots or bulk quantities. This offers customers major advantages, i.e., flexibility of use, choice of buffer in early developmental studies, and avoidance of issues associated with reconstitution of dry powder product obtained from other vendors.
biosyn Immunocyanin in water is a unique formulation and overcomes one of the major drawbacks of using KLH in clinical and commercial grade conjugate vaccine products.
biosyn clinical grade products are extensive tested for safety and lack of viruses including hepatitis A, B, C, vibrio species, rota virus and Norwalk viruses.

 

公司简介

深圳市豪地华拓生物科技有限公司,是一家专业从事于抗体抗原、多肽等产品的研发生产、定制合成和销售的专业高科技技术企业。公司致力于 分子生物学、免疫学、蛋白质组学、细胞生物学技术在科研中的应用与推广,研制方向涉及抗体抗原、多肽、药用辅料、绿色化学、手性化学等领域,面向社会开展产品研制和科技创新活动,为广大企业提供高科技产品,其中部分产品如抗体抗原、化妆品多肽、新型环保的杀菌剂已开始规模生产。 华拓将以弃而不舍、勇于开拓、自主创新、敢为人先的智慧和勇气,在生命科学的道路上,不断为所有从事生物科技研究的同仁们提供 高质量的产品和服务。 李健雄博士,华拓生物 科学家,1984年本科毕业于北京大学,1993年获加拿大西门弗雷泽大学有机化学博士,曾任加拿大维理生物技术有限公司副总裁,加拿大PL实验室 科学家。发表论文50多篇,申请专利20余项。现主要专注生物活性产物的开发及在药物与药物辅料中的应用研究。 2005年1月,在李健雄博士的带领下,广州华拓生物科技有限公司于广州天河高新技术工业园诞生,从事于化妆品多肽、药物多肽、抗体抗原等产品的研发、生产。主要产品包含药物多肽,化妆品多肽,目录多肽,抗体抗原,小分子化合物及其他生物化学试剂。 2008年,我司应客户的需求和扩展市场的需要,我们在深圳成立了深圳市豪地华拓生物科技有限公司。作为生物产品国内外业务的主要业务销售中心,深圳公司全体员工将竭诚为所有客户提供更加*高效的服务。 2013年,为了向更多客户提供更加*的定制服务和产品,我们在武汉设立了新的研发中心和办事处,我们的研发中心得到了在国外具有丰富研发经验的科学家团队的大力支持,研制方向涉及多肽产品、药用辅料、诊断试剂原料等领域,让我们今后推出的产品将更具特色和竞争优势。 华拓生物将根据客户的不同需要提供经济、快速而可靠的产品解决方案,以满足您的研发或生产需要!我们热烈欢迎广大新老客户前来咨询与洽谈,期待着与您的合作! 进入公司首页>

成立时间: 2008-09-25 企业经济性质: 私营独资企业 年营业额:
注册资金: 10万元(人民币) 经营模式: 代理商,生产商, 员工人数: 11-50
公司评价
暂时还没有评价我要评价
该公司其他产品
羊抗牛血清白蛋白抗体检测试剂盒产品图片
羊抗牛血清白蛋白抗体检测试剂盒 价格:面议 产品详情:
  • 价格:面议
  • 品牌:Genemed Synthesis Inc.(GSI)
  • 产地:美国
牛血清白蛋白(BSA)检测试剂盒产品图片
牛血清白蛋白(BSA)检测试剂盒 价格:面议 产品详情:
  • 价格:面议
  • 品牌:Genemed Synthesis Inc.(GSI)
  • 产地:美国
血蓝蛋白相关产品报价
产品标题 价格 产地 公司名称 报价日期  
相关产品热搜词
免责声明:以上所展示的信息由企业自行提供,内容的真实性 、准确性和合法性由发布企业负责,盖德化工网对此不承担任何保证责任。 同时我们郑重提醒各位买/卖家,交易前 请详细核实对方身份,切勿随意打款或发货,谨防上当受骗。如发现虚假信息,请向盖德化工网举报。 点击这里查看防骗指南。
在线询盘
致:深圳市豪地华拓生物科技有限公司
深圳市豪地华拓生物科技有限公司 丁经理
立即聊天!
  求购
  微信
微信扫码联系客服
  公众号
关注微信公众号
  TOP